MICRODETERMINATION OF GLYCOGEN WITH ANTHRONE REAGENT
نویسندگان
چکیده
منابع مشابه
The determination of glycogen in liver and muscle by use of anthrone reagent.
In currently used methods for the determination of glycogen, tissue is extracted either by boiling with 30 per cent potassium hydroxide solution (KOH) or by homogenization with trichloroacetic acid solution (TCA). The glycogen is precipitated from the extract by alcohol and determined either by copper reduction after separation by centrifugation, acid hydrolysis, and neutralization (l-3) or by ...
متن کاملThe Determination of Glycogen in Liver and Muscle by Use of Anthrone Reagent* by Nicholas
In currently used methods for the determination of glycogen, tissue is extracted either by boiling with 30 per cent potassium hydroxide solution (KOH) or by homogenization with trichloroacetic acid solution (TCA). The glycogen is precipitated from the extract by alcohol and determined either by copper reduction after separation by centrifugation, acid hydrolysis, and neutralization (l-3) or by ...
متن کاملThe determination of dextran in blood and urine with anthrone reagent.
Klevas (1) determined dextran in blood by the Hagedorn-Jensen (2) copper reduction method, applied to filtrate prepared by the Somogyi technique (3), after acid hydrolysis of the filtrate for 6 hours. Hint and Thorsen (4) estimated dextran in blood by alkalinizing trichloroacetic acid filtrate, treating the filtrate with standard copper solution, and determining residual copper after 4 hours sh...
متن کاملThe determination of sugar in blood and spinal fluid with anthrone reagent.
The anthrone reagent of Dreywood (1) has been applied to the determination of blood sugar by Durham, Bloom, Lewis, and Mandel (2), Fetz and Petrie (3), and Zipf and Waldo (4). In the procedures developed by these authors, the heat resulting from mixing sulfuric acid with water causes the reaction to take place. Greater precision is obtained by heating the mixture of anthrone, sulfuric acid, and...
متن کاملThe Determination of Sugar in Blood and Spinal Fluid with Anthrone Reagent*
The anthrone reagent of Dreywood (1) has been applied to the determination of blood sugar by Durham, Bloom, Lewis, and Mandel (2), Fetz and Petrie (3), and Zipf and Waldo (4). In the procedures developed by these authors, the heat resulting from mixing sulfuric acid with water causes the reaction to take place. Greater precision is obtained by heating the mixture of anthrone, sulfuric acid, and...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1961
ISSN: 0022-1554,1551-5044
DOI: 10.1177/9.6.670